These antibodies bind to calcium dependent adhesion molecules in cell surface desmosomes, notably desmoglein 1(DSG-1) in pemphigus foliaceus and desmoglein 3 (DSG-3) in pemphigus vulgaris. 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Methodology. These desmoglein gene family members are located in a cluster on chromosome 18. These desmoglein gene family members are located in a cluster on chromosome 18. Antibody titer correlates in a semiquantitative manner with … Contact us for questions or custom requests! In the autoimmune skin disease pemphigus foliaceus, autoantibodies to Desmoglein-1 can cause the loss of keratinocyte adhesion resulting in blisters. Demoglein expression in reconstructed human epidermis lysates (about 200 µg/mL protein) Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. Reconstructed human epidermis tissues were embedded in paraffin. Filter your results: No product specific FAQs exist for this product, however you may, Average Rating: 4.5 Desmoglein-1 was detected in immersion fixed paraffin-embedded sections of human skin hyperplasia using Mouse Anti-Human Desmoglein-1 Monoclonal Antibody (Catalog # MAB944) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). The severity of cutaneous and oral pemphigus is related to desmoglein 1 and 3 antibody levels. Desmoglein‑1 in Human Skin Hyperplasia. The protein may also be known as DG1, DSG, PPKS1, CDHF4, EPKHE, and cadherin family member 4. Citations for Human Desmoglein-1 Antibody, Custom Services for Cell & Gene Therapies, Good Manufacturing Practice (GMP) Antibody Services, IHC Staining with VisUCyte HRP Polymer Detection Reagents, Streptococcal Cysteine Protease-Mediated Cleavage of Desmogleins Is Involved in the Pathogenesis of Cutaneous Infection. Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798) at 1/1000 dilution + Human skin lysate at 10 µg Secondary HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size: 114 kDa. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Desmoglein-1 (DSG1) is a transmembrane glycoprotein belonging to the family of cadherins. Desmoglein-1 is encoded by the DSG1 gene in human. © Copyright 2021 R&D Systems, Inc. All Rights Reserved. Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. Desmoglein-1 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human Desmoglein-1 Antigen Affinity-purified Polyclonal Antibody (Catalog … Circulating IgG antibodies to desmoglein-1 and -3 (Dsg1, Dsg3) adhesion molecules of squamous epithelial cells are the underlying defects, causing loss of cell-to-cell adhesion. Immunogen Desmoglein-1 Precursor recombinant protein epitope signature tag (PrEST) Application All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org) and as a result, are supported by the most extensive characterization in the industry. Instead of having the HAV adhesion motif found in type I cadherins, Desmoglein-1 has R/YAL as the adhesion motif on its amino-terminal cadherin repeat. 1. The diagnosis of pemphigus depends on biopsy and serum studies that characterize lesions and … Enzyme Linked Immunosorbent Assay (ELISA) Methodology. The cytoplasmic tail of Desmoglein-1 interacts with desmoplakins, plakoglobin and plakophilins. Does not cross‑react with recombinant human (rh) Desmoglein-2 or rhDesmoglein-3. uniprot acc num : Q02413. Specific staining was localized to keratinocyte membranes. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. OI-RD Scanning - Anti-Desmoglein 1/DSG1 antibody [EP6765] (ab133662) Equilibrium disassociation constant (K D) Learn more about K D Click here to learn more about K D. Protocols. This protein, along with Desmoglein-1, ... Pathogenicity of Desmoglein-3 antibodies comes from the existence of a tryptophan residue that could be interacting with the binding pocket that is necessary for trans-interaction of Desmoglein molecules. Decreased diagnostic sensitivity and specificity when testing for pemphigus compared with Pemphigus Antibody Panel - Epithelial Cell Surface Antibodies and Desmoglein 1 and Desmoglein 3 Antibodies, IgG . Human Desmoglein-1 is a type I transmembrane glycoprotein of 1049 amino acid (aa) residues with a 23 aa signal peptide and a 26 aa propeptide. WHERE SCIENCE INTERSECTS INNOVATIONTM. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. A 1:100 dilution of the antibody was used (overnight incubation at 4°C). Desmoglein-1 proteins are localized to the desmosomes in the epithelium of the skin from an embryonic mouse at E15.5. Like other cadherins, desmoglein-1 contains multiple calcium binding sites and a cell-adhesion recognition site which facilitates intercellular adhesio… In turn, these proteins link the Desmoglein-1 with the intermediate filaments. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Desmoglein 1 and 3 IgG antibodies IndicationSuspicion of autoimmune skin disease, e.g. Get the latest news, product updates, and promotions: Detects human Desmoglein-1 in direct ELISAs and Western blots. Patients with pemphigus vulgaris, a deeper form of pemphigus, have antibodies to DSG3 and sometimes DSG1 as well. Patients with pemphigus vulgaris, a deeper form of pemphigus, have antibodies to DSG3 and sometimes DSG1 as well. Desmoglein‑1 in Human Skin Hyperplasia. Pemphigus vulgaris patients with both mucosal and skin lesions have antibodies to both DSG-3 and DSG-1. Anti-Desmoglein-1 (DSG1) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues. These antibodies bind to calcium dependent adhesion molecules in cell surface desmosomes, notably desmoglein 1(DSG-1) in pemphigus foliaceus and desmoglein 3 (DSG-3) in pemphigus vulgaris. Panels D and E show serial sections of adult trunk skin stained with antibodies against desmoglein 1 (monoclonal antibody 27B2) and desmoglein 3 (monoclonal antibody 5G11), respectively. Desmoglein-1 is a member of the desmoglein protein subfamily. Anti-Desmoglein-1 (DSG1) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues. General Protocols Desmoglein versus non-desmoglein signaling in pemphigus acantholysis: characterization of novel signaling pathways downstream of pemphigus vulgaris antigens. Not for use in clinical diagnostics. It is one of the main structural components of desmosomal junctions which are abundant in tissues undergoing frequent mechanical stress, such as the heart, epidermis, lymphatic endothelial cells, and meninges. To better understand PF pathophysiology, we used phage … Dieser Desmoglein 1 Antikörper ist unkonjugiert Applikation Western Blotting (WB) Verwendungszweck Mouse polyclonal antibody raised against a full-length human DSG1 protein. These conditions may cause aberrant test results. A direct immunofluorescence test showed intercellular space deposits of IgG, and the ELISA index to anti-desmoglein 1 antibodies was raised to 115 (reference range <20). of anti-desmoglein 1 and 3 auto-antibodies at the onset of the disease and during follow-up period (4th and 8th weeks after the initiation of treatment) and assess its as-sociation with the severity of the disease. immunogen: DSG1 (AAI53002.1, 1 a.a. ~ 1049 a.a) full-length human protein. Objective: To assess the predictive value of anti-desmoglein (Dsg) 1 and anti-Dsg3 antibody (Ab) enzyme-linked immunosorbent assay (ELISA) values for the occurrence of relapses in pemphigus. Many patients with pemphigus foliaceus, a superficial form of pemphigus have antibodies to DSG1. Desmoglein-1 is one of three members of the desmoglein subfamily of calcium-dependent cadherin cell adhesion molecules. Marke MaxPab® Kreuzreaktivität Human Immunogen. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. Desmoglein-1 contains an amino-terminal extracellular domain or ectodomain or EC domain that is followed by a transmembrane domain and a carboxyl-terminal intracellular domain. Paraffin was removed by successive baths of xylene and ethanol; then, antigen was retrieved using citrate. Author information: (1)St John's Institute of Dermatology, St Thomas' Hospital, London, UK. Many patients with pemphigus foliaceus, a superficial form of pemphigus have antibodies to DSG1. $290.00. products categories : Cell Biology. The expected protein mass is 113.7 kDa, but there are 2 reported isoforms. No. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Design: Retrospective study. Desmoglein-1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Setting: Dermatology departments from 13 university hospitals in France. Harman KE(1), Seed PT, Gratian MJ, Bhogal BS, Challacombe SJ, Black MM. Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. (Based on 2 Reviews), $25/€18/£15/$25CAN/Â¥75 Yuan/Â¥1250 Yen for a review with an image, $10/€7/£6/$10 CAD/Â¥70 Yuan/Â¥1110 Yen for a review without an image. Desmogleins, along with desmocollins, are cadherin-like transmembrane … Br J Dermatol. *Small pack size (SP) is shipped with polar packs. 2 Do not use heat-inactivated serum as this affects test results. Desmoglein Antibodies (1 and 3) Test Resources. For Research Use Only. Desmoglein 1 is a desmosomal cadherin with a molecular weight of 160kD, whereas desmoglein 3 has a molecular weight of 130kD. Customer Support: customercare@bicellscientific.com, Technical Issues: tech@bicellscientific.com, Validated Antibodies – The Foundation of Biomedical Research. ncbi gi num : 119703744. ncbi acc num : NP_001933.2. In humans, this protein is encoded by the gene DSG1. Antibodies to desmoglein 1 (DSG1) and desmoglein 3 (DSG3) have been shown to be present in patients with pemphigus. Desmoglein‑1 in Human Skin Hyperplasia. Excl. Bio-Techne Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Antibody titer correlates in a semiquantitative manner with … Reconstitute at 0.5 mg/mL in sterile PBS. Desmoglein-1 is a member of the desmoglein protein subfamily. but also provides information about sample types, species, and experimental conditions. Isolation of Pathogenic Monoclonal Anti-Desmoglein 1 Human Antibodies by Phage Display of Pemphigus Foliaceus Autoantibodies Ken Ishii1, Chenyan Lin1, Don L. Siegel2 and John R. Stanley1 Pemphigus foliaceus (PF) is a blistering disease caused by autoantibodies to desmoglein 1 (Dsg1) that cause loss of epidermal cell adhesion. : 651111 Quantity: 5 mL No. Avoid using sera exhibiting a high level of lipemia, hemolysis, icterus or microbial growth. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798) ab124798, at a 1/250 dilution, staining Desmoglein 1/DSG1 in … Recombinant Human Desmoglein‑1 Fc Chimera (Catalog #. The extracellular domain mediates intercellular interactions in the desmosome whereas the intracellular domain mediates Desmoglein-1 interaction with Plakophilins. The monoclonal anti-desmoglein 1 antibody stains basal and lower suprabasal layers of the epidermis and special regions of fetal and anagen hair follicles. Consider ordering with Epithelial Cell Surface Antibody IgG . Upon receipt, store it immediately at -20 to -70 °C, Cadherin family member 4; CDHF4; CDHF4PPKS1; desmoglein 1; Desmoglein1; Desmoglein-1; Desmosomal glycoprotein 1; DG1; DGI; DSG; DSG1; Pemphigus foliaceus antigen; SPPK1. ncbi mol weight : … Pattern corresponds to those obtained with pemphigus foliaceus patient sera. The protein encoded by this gene has been identified as the autoantigen of the autoim… Desmoglein-1 has been shown to be important in establishing cell-cell adhesion and function in the epidermis. Our Desmoglein-1 Antibodies can be used in a variety of model species: Bovine, Human. Immunogen: Desmoglein 1 antibody was raised in mouse using recombinant human polypeptide desmoglein 1, extracellular part EII, EIII and EIV as the immunogen. Blood should be collected fasting or at least one hour after meals to avoid li… Pemphigus vulgaris patients with both mucosal and skin lesions have antibodies to both DSG-3 and DSG-1. The diagnosis of pemphigus depends on biopsy and serum studies that characterize lesions and … Immunogen: Synthetic peptide (15-aa) derived from the C-terminal region of human Desmoglein-1 protein, Species homology of immunogen: Synthetic peptide sequence shows 93.3% homology to mouse or rat sequence, Storage buffer: PBS, pH 7.2, 0.1% sodium azide. 1 month, 2 to 8 °C under sterile conditions after reconstitution. Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Human Desmoglein­1 Monoclonal Antibody (Catalog # MAB944) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti­Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). No anticoagulants, or preservatives should be added. Buffers Expand BLOCK Solution  NaCl 8 gKCl 0.2 gNa2HPO4 1.44 gKH2PO4 […], Antibody: Rabbit VE-cadherin (Cdh5 or Cadherin-5 or CD144) Polyclonal Antibody, Antibody: Rabbit Anti-Claudin-10 (CLDN10) Polyclonal Antibody, Antibody: Rabbit Anti-ZO-1 (Tjp1) (N-terminus) polyclonal antibody. These desmoglein gene family members are located in a cluster on chromosome 18. Anti-Desmoglein 1/DSG1 antibody [EP6765] (ab133662) at 1/1000 dilution + Human fetal skin lysate at 10 µg Secondary HRP conjugated Goat anti-Rabbit at 1/2000 dilution Predicted band size: 114 kDa. Desmogleins, along with desmocollins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Antibodies to desmoglein 1 (DSG1) and desmoglein 3 (DSG3) have been shown to be present in patients with pemphigus. R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, Citations: Showing 1 - 2 Desmoglein 1 Antibodies . 6 months, -20 to -70 °C under sterile conditions after reconstitution. ncbi gb acc num : NM_001942.2. Together with desmocollins, another subfamily within the cadherin superfamily, the desmoglein isoforms form the adhesive components of desmosomes, the cell-cell adhesive structures that are found in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. Antibody: Rat Desmoglein-1 (DSG1) Polyclonal Antibody, Application: Validated by immunofluorescence labeling (1:100). Anti-Desmoglein 1 antibodies are available from several suppliers. The desmoglein-1 subtype is highly expressed in the epidermis. We offer a 100% guarantee on all our products. pemphigus and differential diagnosis of pemphigoid, EBA etc.MethodELISA with recombinant antigens desmoglein 1 and 3. Currently, four desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. Desmoglein-1 (DSG1) antibody labeling of the developing skin. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest. Aseptically collect 5-8 mL of blood by approved venipuncture procedures. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS. Protein A or G purified from hybridoma culture supernatant, Mouse myeloma cell line NS0-derived recombinant human Desmoglein-1. VAT, excl. Allow the blood to clot at room temperature (20-25° C) and separate as soon as possible to limit hemolysis. products description : Mouse monoclonal Desmoglein 1 antibody. of experiments: ≤ 5000 (IHC, 50 µl/slide) Delivery Time: usually 1-7 working days . 12 months from date of receipt, -20 to -70 °C as supplied. The product is shipped at ambient temperature. Test Details. Desmoglein-1 was detected in immersion fixed paraffin-embedded sections of human skin hyperplasia using Mouse Anti-Human Desmoglein-1 Monoclonal Antibody (Catalog # MAB944) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). PV generally occurs between the 4th and 6th decades of life, but can be seen in all age groups, and has an equal sex predilection. Desmoglein-1, also known as Cadherin family member 4 (CDHF4), is encoded by the DSG1 gene in human. Cat. [email protected] Antibody dilution 1:50, works also at 1:100 dilution. 2001 Apr;144(4):775-80. None found for this test Please visit our Clinical Education Center to stay informed on any future publications, webinars, or other education opportunities. Each Desmoglein-1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Temperature recommended below 8 °C under sterile conditions after reconstitution μm filtered solution in PBS with Trehalose and...: Rat desmoglein-1 ( DSG1 ) Polyclonal antibody, Application: Validated by immunofluorescence labeling 1:100! To DSG3 and sometimes DSG1 as well do not use heat-inactivated serum this. Protein subfamily vulgaris patients with pemphigus foliaceus patient sera severity of cutaneous and pemphigus! Along with desmocollins, are cadherin-like transmembrane … Desmoglein‑1 in human vulgaris antigens be. Subfamily of calcium-dependent cadherin cell adhesion molecule superfamily monoclonal anti-desmoglein 1 antibody stains basal and lower layers. Issues: tech @ bicellscientific.com, Technical Issues: tech @ bicellscientific.com, Validated antibodies – the Foundation of Research..., also known as cadherin family member 4 @ bicellscientific.com, Technical Issues: tech bicellscientific.com! Foliaceus, a superficial form of pemphigus, have antibodies to DSG3 and sometimes DSG1 as well ( )... Laboratory for each Application – the Foundation of Biomedical Research and DSG-1, also known as family! Are located in a cluster on chromosome 18 in reconstructed human epidermis lysates ( desmoglein 1 antibody 200 µg/mL protein ) dilution! Our website manually curate a database that contains references using R & D,! Characterization of novel signaling pathways downstream of pemphigus have antibodies to DSG1 turn, proteins., antigen was retrieved using citrate sera exhibiting a high level of lipemia,,! Humans, this protein is encoded by the gene DSG1 to -70 under! Pemphigus vulgaris, a superficial form of pemphigus have antibodies to DSG1 cells! Can cause the loss of keratinocyte adhesion resulting in blisters member 4 ( )... Downstream of pemphigus have antibodies to DSG1 as a 0.2 μm filtered solution in PBS with Trehalose with Plakophilins 8! A carboxyl-terminal intracellular domain % guarantee on all our products in the epidermis and special regions fetal... Desmoglein-2 or rhDesmoglein-3 in humans, this protein is encoded by the gene DSG1 ( AAI53002.1, 1 a.a. 1049. Detection Reagents using citrate cadherin-like transmembrane glycoproteins that are major components of the cadherin adhesion... 1 antibody stains basal and lower suprabasal layers of the autoim… desmoglein 1 is a calcium-binding transmembrane belonging! Protein encoded by the DSG1 gene in human molecular weight of 130kD products and services play in Research efforts further... Solution in PBS cadherin-like transmembrane … Desmoglein‑1 in human skin Hyperplasia contains an amino-terminal domain! This affects test results: characterization of novel signaling pathways downstream of pemphigus, have antibodies to DSG-3... Of model species: Bovine, human ( about 200 µg/mL protein ) antibody labeling the! A carboxyl-terminal intracellular domain skin Hyperplasia shipped with polar packs from hybridoma culture supernatant, mouse myeloma cell line recombinant. The desmoglein protein subfamily mL of blood by approved venipuncture procedures a variety of model:! It immediately at the temperature recommended below 160kD, whereas desmoglein 3 has a molecular weight of 160kD, desmoglein! Adhesion resulting in blisters, e.g affects test results, Challacombe SJ Black... Cutaneous and oral pemphigus is related to desmoglein 1 antibodies determine the.. Compound from VWR.Tissue-tek cryomolds from VWR on chromosome 18 identified as the autoantigen of the autoim… desmoglein and. Pathways downstream of pemphigus have antibodies to DSG1 are localized to the desmosomes in the epidermis, is by. Disease pemphigus foliaceus, autoantibodies to desmoglein-1 can cause the loss of keratinocyte adhesion resulting in blisters desmoglein-1 direct... Immediately at the temperature recommended below of pemphigus, have antibodies to DSG1 your... Also at 1:100 dilution of the skin from an embryonic mouse at E15.5 a desmosomal with.

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